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Human ISWI chromatin-remodeling complexes sample nucleosomes via transient binding reactions and become immobilized at active sites

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, October 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

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2 patents
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Citations

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Readers on

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121 Mendeley
Title
Human ISWI chromatin-remodeling complexes sample nucleosomes via transient binding reactions and become immobilized at active sites
Published in
Proceedings of the National Academy of Sciences of the United States of America, October 2010
DOI 10.1073/pnas.1003438107
Pubmed ID
Authors

F. Erdel, T. Schubert, C. Marth, G. Langst, K. Rippe

Abstract

Chromatin remodeling complexes can translocate nucleosomes along the DNA in an ATP-dependent manner. Here, we studied autofluorescent protein constructs of the human ISWI family members Snf2H, Snf2L, the catalytically inactive Snf2L+13 splice variant, and the accessory Acf1 subunit in living human and mouse cells by fluorescence microscopy/spectroscopy. Except for Snf2L, which was not detected in the U2OS cell line, the endogenous ISWI proteins were abundant at nuclear concentrations between 0.14 and 0.83 μM. A protein interaction analysis showed the association of multimeric Snf2H and Acf1 into a heterotetramer or higher-order ACF complex. During the G1/2 cell cycle phase, Snf2H and Snf2L displayed average residence times <150 ms in the chromatin-bound state. The comparison of active and inactive Snf2H/Snf2L indicated that an immobilized fraction potentially involved in active chromatin remodeling comprised only 1-3%. This fraction was largely increased at replication foci in S phase or at DNA repair sites. To rationalize these findings we propose that ISWI remodelers operate via a "continuous sampling" mechanism: The propensity of nucleosomes to be translocated is continuously tested in transient binding reactions. Most of these encounters are unproductive and efficient remodeling requires an increased binding affinity to chromatin. Due to the relatively high intranuclear remodeler concentrations cellular response times for repositioning a given nucleosome were calculated to be in the range of tens of seconds to minutes.

Mendeley readers

The data shown below were compiled from readership statistics for 121 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 2%
Netherlands 1 <1%
Switzerland 1 <1%
France 1 <1%
United Kingdom 1 <1%
Germany 1 <1%
Unknown 113 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 35 29%
Student > Ph. D. Student 29 24%
Student > Doctoral Student 11 9%
Student > Master 9 7%
Professor 7 6%
Other 17 14%
Unknown 13 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 57 47%
Biochemistry, Genetics and Molecular Biology 37 31%
Medicine and Dentistry 4 3%
Physics and Astronomy 2 2%
Computer Science 1 <1%
Other 3 2%
Unknown 17 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 May 2013.
All research outputs
#3,093,496
of 17,358,590 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#34,786
of 89,300 outputs
Outputs of similar age
#34,940
of 224,928 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#313
of 799 outputs
Altmetric has tracked 17,358,590 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 89,300 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 30.7. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 224,928 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 799 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.