↓ Skip to main content

PNAS

Human ISWI chromatin-remodeling complexes sample nucleosomes via transient binding reactions and become immobilized at active sites

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, October 2010
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (53rd percentile)

Mentioned by

patent
2 patents
f1000
1 research highlight platform

Citations

dimensions_citation
88 Dimensions

Readers on

mendeley
144 Mendeley
Title
Human ISWI chromatin-remodeling complexes sample nucleosomes via transient binding reactions and become immobilized at active sites
Published in
Proceedings of the National Academy of Sciences of the United States of America, October 2010
DOI 10.1073/pnas.1003438107
Pubmed ID
Authors

Fabian Erdel, Thomas Schubert, Caroline Marth, Gernot Längst, Karsten Rippe

Abstract

Chromatin remodeling complexes can translocate nucleosomes along the DNA in an ATP-dependent manner. Here, we studied autofluorescent protein constructs of the human ISWI family members Snf2H, Snf2L, the catalytically inactive Snf2L+13 splice variant, and the accessory Acf1 subunit in living human and mouse cells by fluorescence microscopy/spectroscopy. Except for Snf2L, which was not detected in the U2OS cell line, the endogenous ISWI proteins were abundant at nuclear concentrations between 0.14 and 0.83 μM. A protein interaction analysis showed the association of multimeric Snf2H and Acf1 into a heterotetramer or higher-order ACF complex. During the G1/2 cell cycle phase, Snf2H and Snf2L displayed average residence times <150 ms in the chromatin-bound state. The comparison of active and inactive Snf2H/Snf2L indicated that an immobilized fraction potentially involved in active chromatin remodeling comprised only 1-3%. This fraction was largely increased at replication foci in S phase or at DNA repair sites. To rationalize these findings we propose that ISWI remodelers operate via a "continuous sampling" mechanism: The propensity of nucleosomes to be translocated is continuously tested in transient binding reactions. Most of these encounters are unproductive and efficient remodeling requires an increased binding affinity to chromatin. Due to the relatively high intranuclear remodeler concentrations cellular response times for repositioning a given nucleosome were calculated to be in the range of tens of seconds to minutes.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 144 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 2%
Germany 1 <1%
Netherlands 1 <1%
Switzerland 1 <1%
United Kingdom 1 <1%
France 1 <1%
Unknown 136 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 37 26%
Student > Ph. D. Student 31 22%
Student > Master 11 8%
Student > Doctoral Student 11 8%
Professor 9 6%
Other 22 15%
Unknown 23 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 54 38%
Biochemistry, Genetics and Molecular Biology 46 32%
Medicine and Dentistry 4 3%
Neuroscience 2 1%
Physics and Astronomy 2 1%
Other 5 3%
Unknown 31 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 May 2013.
All research outputs
#4,640,360
of 24,625,114 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#44,073
of 101,438 outputs
Outputs of similar age
#19,508
of 103,683 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#295
of 689 outputs
Altmetric has tracked 24,625,114 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 101,438 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 38.8. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 103,683 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 689 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.