↓ Skip to main content

PNAS

Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, December 2015
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

news
30 news outlets
blogs
18 blogs
twitter
67 X users
peer_reviews
1 peer review site
facebook
7 Facebook pages
wikipedia
2 Wikipedia pages
googleplus
1 Google+ user
reddit
1 Redditor
video
1 YouTube creator

Citations

dimensions_citation
353 Dimensions

Readers on

mendeley
539 Mendeley
citeulike
1 CiteULike
Title
Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans
Published in
Proceedings of the National Academy of Sciences of the United States of America, December 2015
DOI 10.1073/pnas.1507110112
Pubmed ID
Authors

Jeffrey P. Nguyen, Frederick B. Shipley, Ashley N. Linder, George S. Plummer, Mochi Liu, Sagar U. Setru, Joshua W. Shaevitz, Andrew M. Leifer

Abstract

The ability to acquire large-scale recordings of neuronal activity in awake and unrestrained animals is needed to provide new insights into how populations of neurons generate animal behavior. We present an instrument capable of recording intracellular calcium transients from the majority of neurons in the head of a freely behaving Caenorhabditis elegans with cellular resolution while simultaneously recording the animal's position, posture, and locomotion. This instrument provides whole-brain imaging with cellular resolution in an unrestrained and behaving animal. We use spinning-disk confocal microscopy to capture 3D volumetric fluorescent images of neurons expressing the calcium indicator GCaMP6s at 6 head-volumes/s. A suite of three cameras monitor neuronal fluorescence and the animal's position and orientation. Custom software tracks the 3D position of the animal's head in real time and two feedback loops adjust a motorized stage and objective to keep the animal's head within the field of view as the animal roams freely. We observe calcium transients from up to 77 neurons for over 4 min and correlate this activity with the animal's behavior. We characterize noise in the system due to animal motion and show that, across worms, multiple neurons show significant correlations with modes of behavior corresponding to forward, backward, and turning locomotion.

X Demographics

X Demographics

The data shown below were collected from the profiles of 67 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 539 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 10 2%
Germany 3 <1%
Netherlands 3 <1%
United Kingdom 2 <1%
Japan 2 <1%
Belgium 1 <1%
Hungary 1 <1%
China 1 <1%
Unknown 516 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 162 30%
Researcher 84 16%
Student > Bachelor 53 10%
Student > Master 48 9%
Student > Doctoral Student 27 5%
Other 77 14%
Unknown 88 16%
Readers by discipline Count As %
Neuroscience 119 22%
Agricultural and Biological Sciences 104 19%
Biochemistry, Genetics and Molecular Biology 61 11%
Engineering 48 9%
Physics and Astronomy 40 7%
Other 66 12%
Unknown 101 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 374. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 August 2023.
All research outputs
#84,767
of 25,595,500 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#1,951
of 103,401 outputs
Outputs of similar age
#1,287
of 399,494 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#34
of 846 outputs
Altmetric has tracked 25,595,500 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 103,401 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 39.6. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 399,494 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 846 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.