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Whole-brain calcium imaging with cellular resolution in freely behavingCaenorhabditis elegans

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, December 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

news
30 news outlets
blogs
17 blogs
twitter
76 tweeters
peer_reviews
1 peer review site
facebook
7 Facebook pages
wikipedia
2 Wikipedia pages
googleplus
1 Google+ user
reddit
1 Redditor
video
1 video uploader

Citations

dimensions_citation
201 Dimensions

Readers on

mendeley
449 Mendeley
citeulike
1 CiteULike
Title
Whole-brain calcium imaging with cellular resolution in freely behavingCaenorhabditis elegans
Published in
Proceedings of the National Academy of Sciences of the United States of America, December 2015
DOI 10.1073/pnas.1507110112
Pubmed ID
Authors

Jeffrey P. Nguyen, Frederick B. Shipley, Ashley N. Linder, George S. Plummer, Mochi Liu, Sagar U. Setru, Joshua W. Shaevitz, Andrew M. Leifer

Abstract

The ability to acquire large-scale recordings of neuronal activity in awake and unrestrained animals is needed to provide new insights into how populations of neurons generate animal behavior. We present an instrument capable of recording intracellular calcium transients from the majority of neurons in the head of a freely behaving Caenorhabditis elegans with cellular resolution while simultaneously recording the animal's position, posture, and locomotion. This instrument provides whole-brain imaging with cellular resolution in an unrestrained and behaving animal. We use spinning-disk confocal microscopy to capture 3D volumetric fluorescent images of neurons expressing the calcium indicator GCaMP6s at 6 head-volumes/s. A suite of three cameras monitor neuronal fluorescence and the animal's position and orientation. Custom software tracks the 3D position of the animal's head in real time and two feedback loops adjust a motorized stage and objective to keep the animal's head within the field of view as the animal roams freely. We observe calcium transients from up to 77 neurons for over 4 min and correlate this activity with the animal's behavior. We characterize noise in the system due to animal motion and show that, across worms, multiple neurons show significant correlations with modes of behavior corresponding to forward, backward, and turning locomotion.

Twitter Demographics

The data shown below were collected from the profiles of 76 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 449 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 10 2%
Germany 4 <1%
Netherlands 3 <1%
United Kingdom 2 <1%
Japan 2 <1%
China 1 <1%
Belgium 1 <1%
Hungary 1 <1%
Unknown 425 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 145 32%
Researcher 78 17%
Student > Bachelor 48 11%
Student > Master 45 10%
Student > Doctoral Student 23 5%
Other 63 14%
Unknown 47 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 105 23%
Neuroscience 99 22%
Biochemistry, Genetics and Molecular Biology 48 11%
Engineering 44 10%
Physics and Astronomy 38 8%
Other 58 13%
Unknown 57 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 377. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2021.
All research outputs
#46,572
of 18,011,329 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#1,223
of 90,576 outputs
Outputs of similar age
#1,098
of 378,697 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#29
of 854 outputs
Altmetric has tracked 18,011,329 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 90,576 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 31.8. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 378,697 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 854 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.