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Plasma fibronectin stabilizes Borrelia burgdorferi–endothelial interactions under vascular shear stress by a catch-bond mechanism

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, April 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (61st percentile)

Mentioned by

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31 tweeters
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1 Facebook page
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2 Google+ users

Readers on

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6 Mendeley
Title
Plasma fibronectin stabilizes Borrelia burgdorferi–endothelial interactions under vascular shear stress by a catch-bond mechanism
Published in
Proceedings of the National Academy of Sciences of the United States of America, April 2017
DOI 10.1073/pnas.1615007114
Pubmed ID
Authors

Alexandra F. Niddam, Rhodaba Ebady, Anil Bansal, Anne Koehler, Boris Hinz, Tara J. Moriarty, Niddam, Alexandra F., Ebady, Rhodaba, Bansal, Anil, Koehler, Anne, Hinz, Boris, Moriarty, Tara J.

Abstract

Bacterial dissemination via the cardiovascular system is the most common cause of infection mortality. A key step in dissemination is bacterial interaction with endothelia lining blood vessels, which is physically challenging because of the shear stress generated by blood flow. Association of host cells such as leukocytes and platelets with endothelia under vascular shear stress requires mechanically specialized interaction mechanisms, including force-strengthened catch bonds. However, the biomechanical mechanisms supporting vascular interactions of most bacterial pathogens are undefined. Fibronectin (Fn), a ubiquitous host molecule targeted by many pathogens, promotes vascular interactions of the Lyme disease spirochete Borrelia burgdorferi Here, we investigated how B. burgdorferi exploits Fn to interact with endothelia under physiological shear stress, using recently developed live cell imaging and particle-tracking methods for studying bacterial-endothelial interaction biomechanics. We found that B. burgdorferi does not primarily target insoluble matrix Fn deposited on endothelial surfaces but, instead, recruits and induces polymerization of soluble plasma Fn (pFn), an abundant protein in blood plasma that is normally soluble and nonadhesive. Under physiological shear stress, caps of polymerized pFn at bacterial poles formed part of mechanically loaded adhesion complexes, and pFn strengthened and stabilized interactions by a catch-bond mechanism. These results show that B. burgdorferi can transform a ubiquitous but normally nonadhesive blood constituent to increase the efficiency, strength, and stability of bacterial interactions with vascular surfaces. Similar mechanisms may promote dissemination of other Fn-binding pathogens.

Twitter Demographics

The data shown below were collected from the profiles of 31 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 17%
Unknown 5 83%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 67%
Professor 1 17%
Student > Ph. D. Student 1 17%
Readers by discipline Count As %
Immunology and Microbiology 2 33%
Agricultural and Biological Sciences 1 17%
Physics and Astronomy 1 17%
Medicine and Dentistry 1 17%
Engineering 1 17%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2017.
All research outputs
#426,270
of 8,355,069 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#8,771
of 46,650 outputs
Outputs of similar age
#23,251
of 249,028 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#371
of 978 outputs
Altmetric has tracked 8,355,069 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 46,650 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 24.3. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,028 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 978 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.