| Title |
Light-driven carbon dioxide reduction to methane by nitrogenase in a photosynthetic bacterium
|
|---|---|
| Published in |
Proceedings of the National Academy of Sciences of the United States of America, August 2016
|
| DOI | 10.1073/pnas.1611043113 |
| Pubmed ID | |
| Authors |
Kathryn R. Fixen, Yanning Zheng, Derek F. Harris, Sudipta Shaw, Zhi-Yong Yang, Dennis R. Dean, Lance C. Seefeldt, Caroline S. Harwood |
| Abstract |
Nitrogenase is an ATP-requiring enzyme capable of carrying out multielectron reductions of inert molecules. A purified remodeled nitrogenase containing two amino acid substitutions near the site of its FeMo cofactor was recently described as having the capacity to reduce carbon dioxide (CO2) to methane (CH4). Here, we developed the anoxygenic phototroph, Rhodopseudomonas palustris, as a biocatalyst capable of light-driven CO2 reduction to CH4 in vivo using this remodeled nitrogenase. Conversion of CO2 to CH4 by R. palustris required constitutive expression of nitrogenase, which was achieved by using a variant of the transcription factor NifA that is able to activate expression of nitrogenase under all growth conditions. Also, light was required for generation of ATP by cyclic photophosphorylation. CH4 production by R. palustris could be controlled by manipulating the distribution of electrons and energy available to nitrogenase. This work shows the feasibility of using microbes to generate hydrocarbons from CO2 in one enzymatic step using light energy. |
X Demographics
The data shown below were collected from the profiles of 49 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 8 | 16% |
| United Kingdom | 7 | 14% |
| Spain | 7 | 14% |
| Netherlands | 2 | 4% |
| Australia | 1 | 2% |
| Sweden | 1 | 2% |
| New Zealand | 1 | 2% |
| Denmark | 1 | 2% |
| Germany | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 17 | 35% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 26 | 53% |
| Members of the public | 23 | 47% |
Mendeley readers
The data shown below were compiled from readership statistics for 224 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | <1% |
| Netherlands | 1 | <1% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Portugal | 1 | <1% |
| Denmark | 1 | <1% |
| Belgium | 1 | <1% |
| Japan | 1 | <1% |
| China | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 214 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 59 | 26% |
| Researcher | 38 | 17% |
| Student > Bachelor | 22 | 10% |
| Student > Doctoral Student | 15 | 7% |
| Student > Master | 13 | 6% |
| Other | 33 | 15% |
| Unknown | 44 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 48 | 21% |
| Biochemistry, Genetics and Molecular Biology | 33 | 15% |
| Chemistry | 24 | 11% |
| Environmental Science | 16 | 7% |
| Earth and Planetary Sciences | 15 | 7% |
| Other | 31 | 14% |
| Unknown | 57 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 143. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2016.
All research outputs
#274,813
of 24,625,114 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#5,132
of 101,438 outputs
Outputs of similar age
#5,579
of 350,834 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#90
of 918 outputs
Altmetric has tracked 24,625,114 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 101,438 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 38.8. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 350,834 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 918 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.