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Light-driven carbon dioxide reduction to methane by nitrogenase in a photosynthetic bacterium

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, August 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (98th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Citations

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7 Dimensions

Readers on

mendeley
128 Mendeley
citeulike
1 CiteULike
Title
Light-driven carbon dioxide reduction to methane by nitrogenase in a photosynthetic bacterium
Published in
Proceedings of the National Academy of Sciences of the United States of America, August 2016
DOI 10.1073/pnas.1611043113
Pubmed ID
Authors

Kathryn R. Fixen, Yanning Zheng, Derek F. Harris, Sudipta Shaw, Zhi-Yong Yang, Dennis R. Dean, Lance C. Seefeldt, Caroline S. Harwood, Fixen, Kathryn R, Zheng, Yanning, Harris, Derek F, Shaw, Sudipta, Yang, Zhi-Yong, Dean, Dennis R, Seefeldt, Lance C, Harwood, Caroline S, Fixen KR, Zheng Y, Harris DF, Shaw S, Yang ZY, Dean DR, Seefeldt LC, Harwood CS

Abstract

Nitrogenase is an ATP-requiring enzyme capable of carrying out multielectron reductions of inert molecules. A purified remodeled nitrogenase containing two amino acid substitutions near the site of its FeMo cofactor was recently described as having the capacity to reduce carbon dioxide (CO2) to methane (CH4). Here, we developed the anoxygenic phototroph, Rhodopseudomonas palustris, as a biocatalyst capable of light-driven CO2 reduction to CH4 in vivo using this remodeled nitrogenase. Conversion of CO2 to CH4 by R. palustris required constitutive expression of nitrogenase, which was achieved by using a variant of the transcription factor NifA that is able to activate expression of nitrogenase under all growth conditions. Also, light was required for generation of ATP by cyclic photophosphorylation. CH4 production by R. palustris could be controlled by manipulating the distribution of electrons and energy available to nitrogenase. This work shows the feasibility of using microbes to generate hydrocarbons from CO2 in one enzymatic step using light energy.

Twitter Demographics

The data shown below were collected from the profiles of 56 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 128 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 2%
China 1 <1%
United Kingdom 1 <1%
Denmark 1 <1%
Australia 1 <1%
Japan 1 <1%
Portugal 1 <1%
Belgium 1 <1%
Netherlands 1 <1%
Other 1 <1%
Unknown 117 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 45 35%
Researcher 24 19%
Student > Bachelor 14 11%
Student > Master 11 9%
Student > Doctoral Student 10 8%
Other 24 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 41 32%
Biochemistry, Genetics and Molecular Biology 18 14%
Chemistry 18 14%
Environmental Science 10 8%
Engineering 9 7%
Other 32 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 151. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2016.
All research outputs
#79,748
of 12,267,930 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#1,981
of 77,049 outputs
Outputs of similar age
#3,901
of 264,845 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#81
of 930 outputs
Altmetric has tracked 12,267,930 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 77,049 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 21.8. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,845 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 930 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.