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Quantifying transient binding of ISWI chromatin remodelers in living cells by pixel-wise photobleaching profile evolution analysis

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, November 2012
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  • Above-average Attention Score compared to outputs of the same age (52nd percentile)

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41 Mendeley
Title
Quantifying transient binding of ISWI chromatin remodelers in living cells by pixel-wise photobleaching profile evolution analysis
Published in
Proceedings of the National Academy of Sciences of the United States of America, November 2012
DOI 10.1073/pnas.1209579109
Pubmed ID
Authors

Fabian Erdel, Karsten Rippe

Abstract

Interactions between nuclear proteins and chromatin frequently occur on the time scale of seconds and below. These transient binding events are important for the fast identification of target sites as concluded from our previous analysis of the human chromatin remodelers Snf2H and Snf2L from the imitation switch (ISWI) family. Both ATP-driven molecular motor proteins are able to translocate nucleosomes along the DNA and appear to exert this activity only on a small number of nucleosomes to which they bind more tightly. For mechanistic studies, one needs to distinguish such translocation reactions or other long-lived interactions associated with conformational changes and/or ATP hydrolysis from nonproductive chromatin sampling during target search. These processes can be separated by measuring the duration of nucleosome binding with subsecond time resolution. To reach this goal, we have developed a fluorescence bleaching technique termed pixel-wise photobleaching profile evolution analysis (3PEA). It exploits the inherent time structure of confocal microscopy images and yields millisecond resolution. 3PEA represents a generally applicable approach to quantitate transient chromatin interactions in the 2- to 500-ms time regime within only ∼1 s needed for a measurement. The green autofluorescent protein (GFP)-tagged Snf2H and Snf2L and the inactive Snf2L+13 splice variant were studied by 3PEA in comparison to the isolated GFP or red autofluorescent protein and a GFP pentamer. Our results reveal that the residence time for transient chromatin binding of Snf2H and Snf2L is <2 ms, and strongly support the view that ISWI-type remodelers are only rarely active in unperturbed cells during G1 phase.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 12%
Lithuania 1 2%
France 1 2%
United Kingdom 1 2%
Brazil 1 2%
Netherlands 1 2%
Unknown 31 76%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 37%
Researcher 15 37%
Student > Master 4 10%
Professor 3 7%
Professor > Associate Professor 2 5%
Other 2 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 71%
Biochemistry, Genetics and Molecular Biology 6 15%
Physics and Astronomy 6 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2012.
All research outputs
#2,494,572
of 5,036,908 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#27,170
of 37,938 outputs
Outputs of similar age
#37,065
of 84,599 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#609
of 869 outputs
Altmetric has tracked 5,036,908 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 37,938 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.8. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 84,599 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 869 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.