| Title |
In situ structural analysis of Golgi intracisternal protein arrays
|
|---|---|
| Published in |
Proceedings of the National Academy of Sciences of the United States of America, August 2015
|
| DOI | 10.1073/pnas.1515337112 |
| Pubmed ID | |
| Authors |
Benjamin D Engel, Miroslava Schaffer, Sahradha Albert, Shoh Asano, Jürgen M Plitzko, Wolfgang Baumeister |
| Abstract |
We acquired molecular-resolution structures of the Golgi within its native cellular environment. Vitreous Chlamydomonas cells were thinned by cryo-focused ion beam milling and then visualized by cryo-electron tomography. These tomograms revealed structures within the Golgi cisternae that have not been seen before. Narrow trans-Golgi lumina were spanned by asymmetric membrane-associated protein arrays that had ∼6-nm lateral periodicity. Subtomogram averaging showed that the arrays may determine the narrow central spacing of the trans-Golgi cisternae through zipper-like interactions, thereby forcing cargo to the trans-Golgi periphery. Additionally, we observed dense granular aggregates within cisternae and intracisternal filament bundles associated with trans-Golgi buds. These native in situ structures provide new molecular insights into Golgi architecture and function. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 17% |
| Peru | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 67% |
| Scientists | 1 | 17% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 155 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | <1% |
| Switzerland | 1 | <1% |
| France | 1 | <1% |
| Canada | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 150 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 45 | 29% |
| Researcher | 26 | 17% |
| Student > Master | 16 | 10% |
| Student > Bachelor | 15 | 10% |
| Student > Doctoral Student | 13 | 8% |
| Other | 22 | 14% |
| Unknown | 18 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 56 | 36% |
| Agricultural and Biological Sciences | 55 | 35% |
| Physics and Astronomy | 5 | 3% |
| Chemical Engineering | 4 | 3% |
| Immunology and Microbiology | 3 | 2% |
| Other | 11 | 7% |
| Unknown | 21 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 27. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2022.
All research outputs
#1,473,265
of 25,692,343 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#20,078
of 103,543 outputs
Outputs of similar age
#19,204
of 279,744 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#295
of 873 outputs
Altmetric has tracked 25,692,343 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 103,543 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 39.5. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,744 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 873 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.