| Title |
Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection
|
|---|---|
| Published in |
Proceedings of the National Academy of Sciences of the United States of America, November 2015
|
| DOI | 10.1073/pnas.1506226112 |
| Pubmed ID | |
| Authors |
Rafael D Mesquita, Raquel J Vionette-Amaral, Carl Lowenberger, Rolando Rivera-Pomar, Fernando A Monteiro, Patrick Minx, John Spieth, A Bernardo Carvalho, Francisco Panzera, Daniel Lawson, André Q Torres, Jose M C Ribeiro, Marcos H F Sorgine, Robert M Waterhouse, Michael J Montague, Fernando Abad-Franch, Michele Alves-Bezerra, Laurence R Amaral, Helena M Araujo, Ricardo N Araujo, L Aravind, Georgia C Atella, Patricia Azambuja, Mateus Berni, Paula R Bittencourt-Cunha, Gloria R C Braz, Gustavo Calderón-Fernández, Claudia M A Carareto, Mikkel B Christensen, Igor R Costa, Samara G Costa, Marilvia Dansa, Carlos R O Daumas-Filho, Iron F De-Paula, Felipe A Dias, George Dimopoulos, Scott J Emrich, Natalia Esponda-Behrens, Patricia Fampa, Rita D Fernandez-Medina, Rodrigo N da Fonseca, Marcio Fontenele, Catrina Fronick, Lucinda A Fulton, Ana Caroline Gandara, Eloi S Garcia, Fernando A Genta, Gloria I Giraldo-Calderón, Bruno Gomes, Katia C Gondim, Adriana Granzotto, Alessandra A Guarneri, Roderic Guigó, Myriam Harry, Daniel S T Hughes, Willy Jablonka, Emmanuelle Jacquin-Joly, M Patricia Juárez, Leonardo B Koerich, Angela B Lange, José Manuel Latorre-Estivalis, Andrés Lavore, Gena G Lawrence, Cristiano Lazoski, Claudio R Lazzari, Raphael R Lopes, Marcelo G Lorenzo, Magda D Lugon, David Majerowicz, Paula L Marcet, Marco Mariotti, Hatisaburo Masuda, Karine Megy, Ana C A Melo, Fanis Missirlis, Theo Mota, Fernando G Noriega, Marcela Nouzova, Rodrigo D Nunes, Raquel L L Oliveira, Gilbert Oliveira-Silveira, Sheila Ons, Ian Orchard, Lucia Pagola, Gabriela O Paiva-Silva, Agustina Pascual, Marcio G Pavan, Nicolás Pedrini, Alexandre A Peixoto, Marcos H Pereira, Andrew Pike, Carla Polycarpo, Francisco Prosdocimi, Rodrigo Ribeiro-Rodrigues, Hugh M Robertson, Ana Paula Salerno, Didier Salmon, Didac Santesmasses, Renata Schama, Eloy S Seabra-Junior, Livia Silva-Cardoso, Mario A C Silva-Neto, Matheus Souza-Gomes, Marcos Sterkel, Mabel L Taracena, Marta Tojo, Zhijian Jake Tu, Jose M C Tubio, Raul Ursic-Bedoya, Thiago M Venancio, Ana Beatriz Walter-Nuno, Derek Wilson, Wesley C Warren, Richard K Wilson, Erwin Huebner, Ellen M Dotson, Pedro L Oliveira |
| Abstract |
Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (∼702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods. |
X Demographics
The data shown below were collected from the profiles of 59 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 16 | 27% |
| Spain | 3 | 5% |
| United Kingdom | 3 | 5% |
| Argentina | 3 | 5% |
| Germany | 2 | 3% |
| Brazil | 2 | 3% |
| Ecuador | 1 | 2% |
| Bolivia, Plurinational State of | 1 | 2% |
| Canada | 1 | 2% |
| Other | 4 | 7% |
| Unknown | 23 | 39% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 32 | 54% |
| Members of the public | 25 | 42% |
| Science communicators (journalists, bloggers, editors) | 2 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 417 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 4 | <1% |
| United States | 3 | <1% |
| United Kingdom | 2 | <1% |
| France | 1 | <1% |
| Czechia | 1 | <1% |
| Canada | 1 | <1% |
| Chile | 1 | <1% |
| Spain | 1 | <1% |
| Argentina | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 402 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 71 | 17% |
| Student > Ph. D. Student | 57 | 14% |
| Student > Master | 44 | 11% |
| Student > Bachelor | 44 | 11% |
| Student > Doctoral Student | 29 | 7% |
| Other | 82 | 20% |
| Unknown | 90 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 155 | 37% |
| Biochemistry, Genetics and Molecular Biology | 106 | 25% |
| Medicine and Dentistry | 12 | 3% |
| Immunology and Microbiology | 6 | 1% |
| Environmental Science | 5 | 1% |
| Other | 33 | 8% |
| Unknown | 100 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 80. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2023.
All research outputs
#544,372
of 25,658,139 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#9,482
of 103,524 outputs
Outputs of similar age
#7,368
of 275,195 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#200
of 906 outputs
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